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ocimum basilicum leaf oil

Ocimum basilicum leaf oil is a natural essential oil from basil leaves known for its licorice-like aroma and flavor, commonly used in flavoring and fragrance applications.

General Material Description

Ocimum basilicum leaf oil, commonly called sweet basil leaf oil or simply basil oil, is a natural essential oil derived from the leaves of the Ocimum basilicum plant. This oil is characterized by its clear to pale yellow appearance and a distinct licorice-like odor and flavor profile. Known synonyms include Ocimum basilicum L. herb oil and Oil of Basil. It is extensively used in flavor and fragrance industries due to its aromatic qualities and is classified in flavor databases such as PubChem. The oil is obtained primarily through steam distillation from dried or fresh basil leaves, yielding a complex mixture of aromatic compounds responsible for its sensory notes.

Occurrence, Applicability & Potential Uses

Ocimum basilicum leaf oil is naturally found in the leaves of the sweet basil plant. Its biological origin imparts a unique aromatic profile that is widely valued in flavor and fragrance applications. The oil is employed in the formulation of tonics and cosmetic fragrances, benefiting from its fresh, sweet, and licorice-like sensory attributes. According to FEMA (US) standards, ocimum basilicum leaf oil is recognized for use as a flavoring substance, with specified usage levels to ensure consumer safety. Its application extends to baked goods, beverages, confectionery, and sauces, where it imparts characteristic basil notes without overpowering other flavors.

Physico-Chemical Properties Summary

The oil exhibits moderate solubility in water, with an estimated solubility of approximately 332.1 mg/L at 25°C, indicating partial miscibility useful in formulation. It is a volatile essential oil with a chemical composition that includes components such as linalool and estragole, which contribute to its licorice-like aroma. The oil’s physicochemical nature affects its stability; peroxide formation is a factor during storage, and antioxidants such as BHT or alpha-tocopherol are recommended to maintain quality. These properties influence its handling requirements and suitability for various product preparations in flavor and fragrance development.

FAQ

What is ocimum basilicum leaf oil, and what are its main characteristics?
Ocimum basilicum leaf oil is an essential oil extracted from the leaves of the sweet basil plant. It is distinguished by a licorice-like odor and flavor, imparting sweet, fresh aromatic qualities. The oil is a clear, pale yellow liquid commonly used as a flavoring and fragrance agent. It goes by several names, including sweet basil leaf oil and oil of basil. The oil contains compounds such as linalool and estragole, which contribute to its sensory profile.
How is ocimum basilicum leaf oil sourced and applied in industry?
This oil is obtained primarily via steam distillation of the dried or fresh leaves of the Ocimum basilicum plant. Its natural origin lends it to use in flavoring beverages, baked goods, and sauces, as well as in cosmetic fragrances and tonics. In application, careful control of dosage ensures that the characteristic basil aroma enhances products without overwhelming other notes. Standards such as FEMA (US) provide guidelines for safe usage levels in various product categories.
What safety and regulatory considerations apply to ocimum basilicum leaf oil?
Ocimum basilicum leaf oil is generally recognized as safe when used within recommended limits. Toxicity studies indicate oral LD50 in rats around 1400 mg/kg and dermal LD50 in rabbits over 5000 mg/kg, reflecting low acute toxicity. The IFRA (Global) standards address sensitization concerns by limiting maximum peroxide levels and certain constituent concentrations, such as estragole and methyl eugenol. Additionally, the FEMA Expert Panel evaluates and publishes flavoring assessments to ensure compliance with safety norms in food, beverage, and fragrance applications.

US / EU / FDA / JECFA / FEMA / Scholar / Patents

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Literature & References

ocimum basilicum l. herb oil
Canada Domestic Sub. List:8015-73-4
Pubchem (sid):135362711
Publications by PubMed
Oral supplementation of Ocimum basilicum has the potential to improves the locomotory, exploratory, anxiolytic behavior and learning in adult male albino mice.
The effects of different UV-B radiation intensities on morphological and biochemical characteristics in Ocimum basilicum L.
Variation in essential oil composition within individual leaves of sweet basil (Ocimum basilicum L.) is more affected by leaf position than by leaf age.
Chemical composition and larvicidal activity of essential oil from Ocimum basilicum (L.) against Culex tritaeniorhynchus, Aedes albopictus and Anopheles subpictus (Diptera: Culicidae).
Mosquitocidal and antiplasmodial activity of Senna occidentalis (Cassiae) and Ocimum basilicum (Lamiaceae) from Maruthamalai hills against Anopheles stephensi and Plasmodium falciparum.
Stomatal density and metabolic determinants mediate salt stress adaptation and water use efficiency in basil (Ocimum basilicum L.).
Effect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice.
Photoprotection by foliar anthocyanins mitigates effects of boron toxicity in sweet basil (Ocimum basilicum).
Aqueous and ethanolic leaf extracts of Ocimum basilicum (sweet basil) protect against sodium arsenite-induced hepatotoxicity in Wistar rats.
Partial defoliation and hydraulic integration in Ocimum basilicum (Lamiaceae): testing a model for sectored xylem flow using ¹⁵N labeling.
Characterisation of organic and conventional sweet basil leaves using chromatographic and flow-injection mass spectrometric (FIMS) fingerprints combined with principal component analysis.
Effect of climate change on Alternaria leaf spot of rocket salad and black spot of basil under controlled environment.
Phenylpropanoid biosynthesis in leaves and glandular trichomes of basil (Ocimum basilicum L.).
Antibacterial properties of essential oils and methanol extracts of sweet basil Ocimum basilicum occurring in Bangladesh.
Chemomodulatory efficacy of basil leaf (Ocimum basilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen-induced skin and forestomach papillomagenesis.
Ocimum basilicum L.: phenolic profile and antioxidant-related activity.
Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L.
Light suppresses sporulation and epidemics of Peronospora belbahrii.
Epidermal coumaroyl anthocyanins protect sweet basil against excess light stress: multiple consequences of light attenuation.
Evaluation of antileishmanial activity of South Indian medicinal plants against Leishmania donovani.
Root internalization, transport and in-planta survival of Salmonella enterica serovar Newport in sweet basil.
Anthocyanin inheritance and instability in purple basil (Ocimum basilicum L.).
In vitro rosmarinic acid accumulation in sweet basil (Ocimum basilicum L.).
Light reflected from colored mulches affects aroma and phenol content of sweet basil (Ocimum basilicum L.) leaves.
Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese.
Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid.
Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran.
4-coumarate: CoA ligase partitions metabolites for eugenol biosynthesis.
Deployment of municipal solid wastes as a substitute growing medium component in marigold and basil seedlings production.
In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum.
Use of a common laboratory glassware detergent improves recovery of Cryptosporidium parvum and Cyclospora cayetanensis from lettuce, herbs and raspberries.
Evaluation of the gastric antiulcerogenic effects of Solanum nigrum, Brassica oleracea and Ocimum basilicum in rats.
Biosynthesis of estragole and methyl-eugenol in sweet basil (Ocimum basilicum L). Developmental and chemotypic association of allylphenol O-methyltransferase activities.
The formulation of an effective topical antibacterial product containing Ocimum gratissimum leaf essential oil.
Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum.
Guard cell apoplastic photosynthate accumulation corresponds to a phloem-loading mechanism.
Rapid staining method to detect and identify downy mildew (Peronospora belbahrii) in basil.
Protection of cadmium chloride induced DNA damage by Lamiaceae plants.
Interaction of Salmonella enterica with basil and other salad leaves.
An investigation of the storage and biosynthesis of phenylpropenes in sweet basil.
Characterization of geraniol synthase from the peltate glands of sweet basil.
Antidiarrhoeal activities of Ocimum gratissimum (Lamiaceae).
Antimelanoma and radioprotective activity of alcoholic aqueous extract of different species of Ocimum in C(57)BL mice.
Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines.
Randomized placebo-controlled, single blind trial of holy basil leaves in patients with noninsulin-dependent diabetes mellitus.
Phylogeny and evolution of basils and allies (Ocimeae, Labiatae) based on three plastid DNA regions.
Enantiomeric composition of (3R)-(-)- and (3S)-(+)-linalool in various essential oils of Indian origin by enantioselective capillary gas chromatography-flame ionization and mass spectrometry detection methods.
Folklore therapeutic indigenous plants in periodontal disorders in India (review, experimental and clinical approach).
Activity of essential oils and individual components against acetyl- and butyrylcholinesterase.
Study of ambient ozone phytotoxicity in Ukraine and ozone protective effect of some antioxidants.

Other Information

FDA Substances Added to Food (formerly EAFUS):View
Typical G.C.
Wikipedia:View
FAO:BASIC PRINCIPLES OF STEAM DISTILLATION

General Material Information

Preferred name ocimum basilicum leaf oil
Trivial Name Oils, basil
Short Description sweet basil leaf oil
CAS Number 8015-73-4
FEMA Number 2119
FDA UNII Search
MDL MFCD00217166
FDA Patent No longer provide for the use of these seven synthetic flavoring substances
FDA Mainterm 8015-73-4 ; BASIL, OIL (OCIMUM BASILICUM L.)
Synonyms
  • sweet basil leaf oil
  • ocimum basilicum l. herb oil
  • Oils, basil
  • Basil oil
  • Oil of Basil
  • Ocimum basilicum oil

PhysChem Properties

Material listed in food chemical codex No
Solubility
water, 332.1 mg/L @ 25 °C (est) Yes

Organoleptic Properties

Odor Type: Licorice
basil, herbal, tarragon
General comment At 100.00 %. basil
Flavor Type: Licorice
basil, licorice, tarragon
General comment Basil

Occurrences

Potential Uses

Applications
Flavoring purposes Tonic
Cosmetic purposes Fragrance , Tonic

Safety Information

Safety information

Hazards identification
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
Pictogram
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
oral-rat LD50 1400 mg/kg
Food and Cosmetics Toxicology. Vol. 11, Pg. 867, 1973.

Dermal Toxicity:
skin-rabbit LD50 > 5000 mg/kg
Food and Cosmetics Toxicology. Vol. 11, Pg. 867, 1973.

Inhalation Toxicity:
Not determined

Safety in use information

Category:
flavor and fragrance agents
RIFM Fragrance Material Safety Assessment: Search
IFRA Code of Practice Notification of the 49th Amendment to the IFRA Code of Practice
IFRA Critical Effect:
Sensitization
IFRA fragrance material specification:
Linalool and natural products known to be rich in linalool, such as bois de rose, coriander or ho wood oil, should only be used when the level of peroxides is kept to the lowest practical level. It is recommended to add antioxidants at the time of production of the raw material. The addition of 0.1% BHT or alpha-tocopherol for example has shown great efficiency. The maximum peroxide level for products in use should be 20 mmol/l.
contains the following IFRA (Annex) restricted components: (non-analysis max. level reference only)
estragol Max. Found:
eugenol Max. Found:
methyl eugenol Max. Found:
para-anisaldehyde Max. Found: 0.1 % and Reason: Sensitization
Use levels for FEMA GRAS flavoring substances on which the FEMA Expert Panel based its judgments that the substances are generally recognized as safe (GRAS).
The Expert Panel also publishes separate extensive reviews of scientific information on all FEMA GRAS flavoring substances and can be found at FEMA Flavor Ingredient Library
publication number: 3
Click here to view publication 3
average usual ppmaverage maximum ppm
baked goods: -4.20000
beverages(nonalcoholic): -2.00000
beverages(alcoholic): --
breakfast cereal: --
cheese: --
chewing gum: --
condiments / relishes: -15.00000
confectionery froastings: --
egg products: --
fats / oils: --
fish products: --
frozen dairy: -2.70000
fruit ices: -2.70000
gelatins / puddings: -0.01000
granulated sugar: --
gravies: --
hard candy: -6.20000
imitation dairy: --
instant coffee / tea: --
jams / jellies: --
meat products: -24.00000
milk products: --
nut products: --
other grains: --
poultry: --
processed fruits: --
processed vegetables: --
reconstituted vegetables: --
seasonings / flavors: --
snack foods: --
soft candy: --
soups: --
sugar substitutes: --
sweet sauces: --

Safety references

EPI System: View
Chemical Carcinogenesis Research Information System:Search
AIDS Citations:Search
Cancer Citations:Search
Toxicology Citations:Search
EPA Substance Registry Services (TSCA):8015-73-4
EPA ACToR:Toxicology Data
EPA Substance Registry Services (SRS):Registry
National Institute of Allergy and Infectious Diseases:Data
ocimum basilicum l. herb oil
Chemidplus:0008015734
RTECS:CR3325000 for cas# 8015-73-4